Repository: Freie Universität Berlin, Math Department

Nanoscopic compartmentalization of membrane protein motion at the axon initial segment

Albrecht, D. and Winterflood, C. M. and Sadeghi, M. and Tschager, T. and Noé, F. and Ewers, H. (2016) Nanoscopic compartmentalization of membrane protein motion at the axon initial segment. J. Cell Biol., 215 . pp. 37-46.

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Abstract

The axon initial segment (AIS) is enriched in specific adaptor, cytoskeletal, and transmembrane molecules. During AIS establishment, a membrane diffusion barrier is formed between the axonal and somatodendritic domains. Recently, an axonal periodic pattern of actin, spectrin, and ankyrin forming 190-nm-spaced, ring-like structures has been discovered. However, whether this structure is related to the diffusion barrier function is unclear. Here, we performed singleparticle tracking time-course experiments on hippocampal neurons during AIS development. We analyzed the mobility of lipid-anchored molecules by high-speed single-particle tracking and correlated positions of membrane molecules with the nanoscopic organization of the AIS cytoskeleton. We observe a strong reduction in mobility early in AIS development. Membrane protein motion in the AIS plasma membrane is confined to a repetitive pattern of ∼190-nm-spaced segments along the AIS axis as early as day in vitro 4, and this pattern alternates with actin rings. Mathematical modeling shows that diffusion barriers between the segments significantly reduce lateral diffusion along the axon.

Item Type:Article
Subjects:Physical Sciences > Physics
Mathematical and Computer Sciences > Mathematics > Mathematical Modelling
Biological Sciences > Biology > Cell Biology
Divisions:Department of Mathematics and Computer Science > Institute of Mathematics > Comp. Molecular Biology
ID Code:1949
Deposited By: BioComp Admin
Deposited On:13 Sep 2016 22:59
Last Modified:27 Jun 2017 14:55

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